| Gel : | 12.5% Polyacrylamide Gel (Laemli) |
| Buffer solution : | Tris/Glycine buffer solution |
| Electrophoresis : | 30 mA constant current, 50min |
| Rapid Stain CBB Kit (Coomassie R-250) | ||
| Quick: | staining and destaining are completed within 30 minutes | |
| Simple: | just mix the kit components with purified water | |
| Highly sensitive: | detect as little as 20 ng protein | |
| Application |  |
|
| Composition | A solution : contains acetic acid
B solution : contains ethanol and Coomassie Brilliant Blue R-250 |
|
| Human Serum | |
| Lane1 : marker | Lane 7 : 250 ng |
| Lane2 : 5,000 ng | Lane 8 : 100 ng |
| Lane3 : 2,500 ng | Lane 9 : 70 ng |
| Lane4 : 1,000 ng | Lane10 : 40 ng |
| Lane5 : 750 ng | Lane11 : 20 ng |
| Lane6 : 500 ng | Lane12 : marker |