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Protease Inhibitor Cocktail (EDTA free)
Inhibition of intra and extra cellular proteases is vital to purify and collect the expressed proteins. Saving trouble of finding adequate inhibitors, a wide range of proteases are inhibited by the Protease Inhibitor Cocktail. This product does not interfere with metalloproteins since it is EDTA free.
  • The product does not contain phosphorylated proteins.
  • The product is phosphatase free.
  • The preservative in the product does not affect the enzyme activity of horseradish peroxidase (HRP).
  • Store the product in refrigerator after opening.
    		•
    Wide range: Contains inhibitors for a variety of serin and cysteine proteases
    Contain no EDTA: efficient for purification of metalloproteins and His tag gene exchanged proteins
    Easy to use: dilute according to your need (100X stock concentration)
    Application Enzyme inhibition efficiency

     

    Origin of Protease Protease Protease Inhibitor Cocktail (EDTA free) (Protease concentration)
    Papain
    (from papaya)    		 Cystein 100%
    (2.5 µg/ml)    		 96.1%
    (5.0 µg/ml)    		 96.5%
    (20 µg/ml)
    Trypsin
    (from porcine pancreas)    		 Serine 96.9%
    (2.0 µg/ml)    		 94.0%
    (4.0 µg/ml)    		 88.1%
    (8.0 µg/ml)
    Chymotrypsin
    (from bovine pancreas)    		 Serine 95.1%
    (0.75 µg/ml)    		 90.5%
    (1.5 µg/ml)    		 83.1%
    (3.0 µg/ml)
    Pronase
    (from Streptomyces griseus)    		 Mixture 96.5%
    (0.6 µg/ml)    		 95.3%
    (1.2 µg/ml)    		 92.3%
    (2.4 µg/ml)
    Pancreas extract
    (bovine)    		 Mixture 89.8%
    (5.0 µg/ml)    		 87.2%
    (10 µg/ml)    		 85.6%
    (20 µg/ml)
    Composition The product contains:

     

    Inhibitors Targeted proteasee
    50 mmol/l 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) Serine protease
    15 µmol/l Aprotinin Serine protease, elastase
    100 µmol/l E-64 Cystein protease
    100 µmol/l Leupeptin hemisulfate monohydrate Cystein protease, trypsin like protease
    Protocol
  • Dissolve the powdered product by adding 1 ml deionized water into the bottle to make the 100 times concentrated stock solution.
  • Dilute with your choice of buffer to make 100 ml of 1X protease inhibitor solution.
    		•
    Caution
  • In case of very high protease concentration, use higher concentration of the product (dilute only up to between 50 and 20 times).
  • Keep the inhibitor cocktail stock solution at -20? or lower, and use it within 3 months.
    		•
    Expiration For powder in unopened bottle: two years from manufacturing. Expiration date is stated on the product label (Exp. yy / mm)
    Protease Inhibitor Cocktail for General Use (100X)
    Features
  • The product contain 5 kinds of protease inhibitors for different targets.
  • The product is 100 times concentrated stock solution. Dilute with any buffer of your choice.
    		•
    Composition The product contains:

     

    Inhibitors Targeted proteasee
    50 mmol/l 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) Serine protease
    15 µmol/l Aprotinin from bovine lung Serine protease, elastase
    100 µmol/l E-64 Cystein protease
    100 µmol/l Leupeptin hemisulfate monohydrate Cystein protease, trypsin like protease
    50 µmol/l Disodium dihydrogen ethylenediaminetetraacetate dihydrate Metallo protease
    Protocol
  • Dissolve the powdered product by adding 1 ml deionized water into the bottle to make the 100 times concentrated stock solution.
  • Dilute with your choice of buffer to make 100 ml of 1X protease inhibitor solution.
    		•
    Caution
  • In case of very high protease concentration, use higher concentration of the product (dilute only up to between 50 and 20 times).
  • Keep the inhibitor cocktail stock solution at -20? or more, and use it within 3 months.
    		•
    Expiration For powder in unopened bottle: two years from manufacturing. Expiration date is stated on the product label (Exp. yy / mm)

     

    Protease Inhibitor Cocktail for Use with Mammalian Cell and Tissue Extracts
    Features
  • The product contain 6 kinds of protease inhibitors for different targets.
  • The product is a concentrated type. Dilute with any buffer of your choice.
    		•
    Composition The protease inhibitors are mixed in Dimethyl sulfoxide (DMSO)

     

    Inhibitors Targeted proteasee
    100 mmol/l 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) Serine protease
    80 µmol/l Aprotinin from bovine lung Serine protease, elastase
    1.5 mmol/l E-64 Cystein protease
    2 mmol/l Leupeptin hemisulfate monohydrate Cystein protease, trypsin like protease
    5 mmol/l Bestatin Aminopeptidase
    1 mmol/l Pepstatin A Asparticprotease
    Protocol Dilute 50 - 100 times with any buffer of your choice
    Caution In case of very high protease concentration, use higher concentration of the product
    Expiration One year from manufacturing. Expiration date is stated on the product label (Exp. yy / mm)
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