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10901 Amino-dPEG 36-t-butyl ester

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CAS: 872340-65-3

Item Name: Amino-dPEG 36-t-butyl ester

Item #: 10901

Mol. Wt.: 1731.09;

single compound dPEG Spacer is 111 atoms and 132.7 A

10901 Amino-dPEG 36-t-butyl ester

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CAS: 872340-65-3
Item Name: Amino-dPEG 36-t-butyl ester
Item #: 10901
Mol. Wt.: 1731.09;
single compound dPEG Spacer is 111 atoms and 132.7 A
Product Features and Benefits: A carboxy protected dPEG x amino acid pegylation reagent dPEG pegylation spacer and modifier with a reactive amine and a t-butyl protected carboxylic acid. dPEG pegylation spacer is extremely hydrophilic, will enhance the water solubility of a compound in which it is incorporated and reduce non-specific binding where applicable. The spacer is also non-immunogenic. The ester is both water soluble, as well as in common used organic solvents of moderate prolarity. t-Butyl group is easily removed with TFA (25% TFA in CH2Cl2, ice bath/0oC, takes about 5 h) t-Butyl group provides a potentially powerful purification handle due to its hydrophobicity. Subsequently it is removed to perform additional chemistry. This applies best when using normal phase (e.g., silica gel) chromatography. t-Butyl group neutralizes the zwitterion of the amino acid for better reactivity at the amine Reacts with carboxylic acids and other carbonyls Protocols: The following are general use guidelines for reaction with: a. Reaction with an active ester: Find a solvent in which active ester is soluble (methylene chloride is a preferred solvent) and dissolve the ester in the dry solvent, add triethylamine, then add the amine, either neat or drop wise as a solution in the reaction solvent. Use at least a 10% excess of all reagents relative to the active ester. This can be carried out at ambient temperatures. b. Reaction with a free acid: generate the active ester in situ with EDC and NHS or HOBt, then add the triethylamine, followed by the amino-dPEG4 t-butyl ester; or ii) combine the acid, NHS or HOBt, TEA, and the amino-dPEG