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10014 TRIS(2-carboxyethyl)phosphine hydrochloride (TCEP)

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CAS: 58626-38-3

Item Name: TRIS(2-carboxyethyl)phosphine hydrochloride (TCEP)

Item #: 10014

Mol. Wt.: 286.65

10014 TRIS(2-carboxyethyl)phosphine hydrochloride (TCEP)

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$27.50

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CAS: 58626-38-3
Item Name: TRIS(2-carboxyethyl)phosphine hydrochloride (TCEP)
Item #: 10014
Mol. Wt.: 286.65;
single compound
Product Features and Benefits: great reducing agent for disulfides A water soluble, odorless phosphine capable of rapid, specific reduction of disulfides to thiols (see reference 2 below),even stable disulfides that are normally resistant to reduction. More stable and more effective than DTT (Cleland's reagent) Selective for disulfides Resistant to air oxidation Very stable in aqueous buffers (except phosphate buffers; see below) at almost any pH. Can be made and stored as 10x stock solutions and diluted to the working concentration just prior to use Stable in aqueous, acidic, and basic solutions from pH 1.5 to pH 9. The pH of TCEP dissolved in water is about 2.5 Product and Handling and stablitiy: An easy-to-handle solid sold as the HCL salt. Soluble in water to 310 mg/mL. Not soluble in most organic solvents. Whenever possible, reactions with TCEP should run in water or in aqueous buffer Although TCEP is resistant to air oxidation, it is a little bit hygroscopic, and we recommend that customers take appropriate precautions in handling the compound TCEP is not very stable in phosphate buffers, especially at neutral pH. If TCEP is to be used with a phosphate bufffer, it should be made up just prior to use. Stock solutions of this material cannot be made and stored. Not compatible with isoelectric focusing techniques, because the product is charged in solution. Procedure: Hermanson (see reference below) has procedures for TCEP reduction of disulfides in proteins. Other procdures may be found throughout the literature. In general TCEP reduction od disulfides is carried out in water or in aqueous buffer at a molar concentration of 3:1 (or larger) TCEP:disulfide. However, for disulfide reduction of proteins, a TCEP concentration of 20 mM and 50 mM is sufficient for complete, rapid range from room temperature to 100oC, depending on the disulfide being reduced. Incubation times range from a few minutes to 1-2 hours. Each reduction must be optimized for the particular disulfide(s) being reduced.
Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), paged 95-97 (protcol), 654 Burns, et al., Selective reduction of disulfides by tris(2-carboxyethyl)phosphine, Journal of Organic Chemistry, 1991, 56, 2648-2650 Han and Han, A procedure for quantitative determination of Tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol, Analytical Biochemistry, 1994, 220, 5-10 Cline, et al., New water-soluble phosphines as reductions of peptide and protein disulfide bonds: reactivity and membrane permeability, Biochemistry, 2004, 43, 15195-15203 Satoh, et al., Simultaneous determination of -lipoic acid and its reduced form by high-performance liquid chromatography with fluorescence detention, Journal of Chromatography B, 2007, 854, 109-115 Toyo'oka. Recent advances in seperation and detection methods for thiol compounds in biological samples, Journal of Chromatography B, 2009, 877, 3318-3330