Advanced Search   

Customer Service info@echemshop.com +1-302-292-8500

10010 Biotin-dPEG 4-PFP ester

Quick Overview

CAS: 1334172-58-5

Item Name: Biotin-dPEG 4-PFP ester

Item #: 10010

Mol. Wt.: 657.65;

single compound dPEG Spacer is 16 atoms and 19.2 A

10010 Biotin-dPEG 4-PFP ester

* Required Fields

$80.00

Details

CAS: 1334172-58-5
Item Name: Biotin-dPEG 4-PFP ester
Item #: 10010
Mol. Wt.: 657.65;
single compound dPEG Spacer is 16 atoms and 19.2 A
Product Features and Benefits: Amine reactive pegylation biotinylation reagent with a dPEG 4 spacer arm Optimal for solid phase synthesis, as alternative to the NHS ester (10200) Very water soluble, hydrophilic and eliminates non-specific binding upon labeling. Ideal spacer length for binding streptavidin conjugates; chain length from amide to terminal carbonyl is 19.2 Angstroms. Designed to be the same length as the LC-LC spacer, which is considered an ideal length for the streptavidin binding pocket. Non-antigenic and non-immunogenic spacer arm Application: ELIMINATES aggregation and precipitation when labeling antibodies and other biological materials (a severe problem with conventional biotinylation reagents)
Protocol: See Hermanson (reference), pg. 728 for the NHS-dPEG 4 biotin. While the NHS ester can be used directly in water, this should, as the protocol calls, be dissolved in an organic solvent for optimal and easiest application.
Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds.