Staining
| Gel : | ReadyGel 4-20% |
| Buffer solution : | Tris/Glycine/SDS buffer solution (400ml) |
| Electrophoresis : | 80 mA (constant voltage) |
| Staining : | 1 hr. |
| CBB Stain One (Ready To Use) (Coomassie G-250) | ||
| Ready to use : | one solution in one bottle | |
| Fast and convenient : | use only water for destaining | |
| Safe : | no hazardous methanol or acetic acid | |
| Highly sensitive : | detects proteins at nanogram level | |
| Application |  |
|
| Human Serum | |
| Lane1 : E coli proteins (SDS, with 2ME buffer extraction) 5 µl | |
| Lane2 : E coli proteins (SDS, with 2ME buffer extraction) (10 time dilution) 5 µl | |
| Lane3: protein markers (1.7 µg/µl) 2 µl | |
| Lane4: protein markers (550 ng/µl) 2 µl | |
| Lane5: protein markers (170 ng/µl) 2 µl | |
| Lane6: protein markers (55 ng/µl) 2 µl | |