Staining
Gel : | ReadyGel 4-20% |
Buffer solution : | Tris/Glycine/SDS buffer solution (400ml) |
Electrophoresis : | 80 mA (constant voltage) |
Staining : | 1 hr. |
CBB Stain One (Ready To Use) (Coomassie G-250) | ||
Ready to use : | one solution in one bottle | |
Fast and convenient : | use only water for destaining | |
Safe : | no hazardous methanol or acetic acid | |
Highly sensitive : | detects proteins at nanogram level | |
Application | ![]() |
Human Serum | |
Lane1 : E coli proteins (SDS, with 2ME buffer extraction) 5 µl | |
Lane2 : E coli proteins (SDS, with 2ME buffer extraction) (10 time dilution) 5 µl | |
Lane3: protein markers (1.7 µg/µl) 2 µl | |
Lane4: protein markers (550 ng/µl) 2 µl | |
Lane5: protein markers (170 ng/µl) 2 µl | |
Lane6: protein markers (55 ng/µl) 2 µl |