Details
| CAS: 295329-79-2 |
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| Item Name: Biotin-dPEG 3-cyanocobalamin |
| Item #: 10218 |
| Mol. Wt.: 473.61; |
| single compound dPEG Spacer is 15 atoms and 18.1 A |
| Product Features and Benefits: Biotin reagent for analytical measurements of biotin binding to streptavidin for other biotinylated species. The reagent has been uniquely designed as a competitive standard reagent to assess the relative binding of biotin derivatives with avidin and streptavidin (on/off rate determinations) No other comparable reagent is available anywhere else. dPEG reagent contains a 3 unit pegylation chain for optimal binding distance and physical properties to balance to incredible hydrophilicity of biotin. Designed by Dr. Scott Wilbur at the University of Washington (see references) Applications and protocols: Please refer to the following publications by Scott Wilbur for details on using this reagent. |
| References: D. Scott Wilbur, et. al., Biotin Reagents for Antibody Pretargeting. 4. Selection of Biotin Conjugates for in Vivo Application Based on Their Dissociation Rate from Avidin and Streptavidin, Bioconjugate Chemistry, 11, 569-583 (2000) D. Scott Wilbur, et. al., Evaluation of Biotin-Dye conjugates for Use in an HPLC Assay To Assess Relative Binding of Biotin Derivatives with Avidin and Streptavidin, Bioconjugate Chemistry, 11, 584-598 (2000). |
