Biotin-dPEG®x-MAL

Amount: 25mg

Item Name: Biotin-dPEG®23-MAL

Item #: 10785

Mol. Wt.: 1450.72;

single compound dPEG® Spacer is 77 atoms and 94.1 A

Product Features and Benefits: Sulfhydryl reactive biotinylation reagent with dPEG®x pegylation spacers! ONLY commercially available sulfhydryl reactive biotinylation reagents with hydrophilic spacer and maleimide functionality. dPEG® pegylation spacers provide HUGE practical advantages. Conventional thiol reactive biotinylation reagents are far too hydrophobic to be of practical commercial use; causing inherently insurmountable problems with solubility, aggregation and precipitation of biotinylated species. Many of these disadvantages have not been apparent due to the lacit of alternatives . . . UNTIL NOW! Advantages: The dPEG® pegylation spacer makes the reagent water soluble, and the remarkable properties of the dPEG® aids in eliminates non-specific binding problem in the application due to the biotin and spacer combination, increasing S/N. These are often insurmountable barriers with conventional biotinylation reagents; spacer allows the vital biotin to stick out into the aqueous environment, and makes it more available then conventional reagents to binding with Streptavidin. Biotinylates at acidic to neutral pH, due to the incredibly high reactivity of the maleimide with thiols/sulfhydryls. Longer spacer may allow biotin accessibility of the biotin by the streptavidin/avidin conjugate binding system where the reactive thiols are buried in the labeled system, away from the surface.. Pegylation arm is non-antigenic and non-immunogenic In solution there are no aggregation or precipitation problems when labeling antibodies and other biological materials (a known problem with conventional biotinylation reagents) Applications: Biotinylation of a) sulfhydryl containing peptides and proteins; b) terminal sulfhydryl modified DNA Protocols &

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). See pp. 732-733 in Greg’s new edition for a summary of the reactions, plus a typical protocol.