FlashTube 2002 with 2.0g of...

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1. FlashTube 2002 with 2.0g of silica (1 box of 20 tubes)

2. FlashTube 2002 with 2.0g of silica (5 boxes, 20 tubes each)

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eChemShop - Chemical Reagents, Natural Compounds, Software, HPLC columns :: Flash Chromatography :: FlashTube 2002 with 2.0g of silica (1 box of 20 tubes)

FlashTube 2002 with 2.0g of silica (1 box of 20 tubes)

FlashTubeTM chromatography is a novel, patented (US patent 6,423,220) concept for rapid and efficient purification of chemical libraries.

FlashTubes are made of polyethylene and are packed with TLC silica 6-35µm containing a fluorescence indicator.

* Puts parallel purification in the hands of every synthetic chemist.
* Minimizes the time needed for purification. More libraries!
* More than 25 separations per hour.
* Pure compounds enhances the quality of your libraries.
* Reduced solvent use compared to normal chromatography.
* Can be used for all type of compounds.
* Utilizes up to five times less solvent than normal chromatography
* Comes pre-packed with silica

FlashTube chromatography puts parallel purification in your own hands. It is simple and rapid, almost like running TLC. Below are some hints and advice when using it.

Type of compounds

All type of compounds which can be separated on silica TLC and which absorb UV-light can be purified by FlashTube chromatography.

Finding a solvent system for elution

Usually a TLC system which separates the desired
compound(s) can be used for FlashTube chromatography. Some examples are given below. Unlike normal chromatography relatively high Rf-values (~0.5) can be used.

Adding the sample

The top of the column is wetted with the eluting solvent before adding the sample. This may be dissolved in the same or sometimes a less polar solvent. The rest of the solvent needed for the separation is finally added. The total amounts of solvent which can be absorbed on the columns are: FlashTube 2002: 2.5 ml. FlashTube chromatography utilizes up to five times less solvent than normal chromatography!
Compounds which are poorly soluble in non-polar solvents may be dissolved in small amounts of a polar solvent and added to the column. The polar solvents are then removed under vacuum prior to addition of the eluting solvent.

Cutting the column

When the column has been developed the desired bands are located in UV-light and cut out with the FlashTube cutter. To avoid band broadening this should be done relatively soon. As an alternative to cutting out a piece of the column, the silica may be carved out from one end of the band.

Isolating the compounds

Different procedures may be used.
Extraction/Separation. The column piece is placed in a test tube and the plastic is removed. For extraction a relatively polar solvent (solvent combination) is used and the silica may be centrifuged down. Alternatively the silica is treated with a water solution (optionally containing acid or base) and a low density solvent added to extract the compound into the organic phase which is finally separated.
Filtration. The column piece that has been cut out is placed in a reservoir equipped with a filter (10µ) and a valve. The plastic tube is removed and the silica is extracted with a relatively polar solvent (solvent combination). The silica may alternatively be treated with a water solution (optionally containing acid or base) and chloroform added to extract the compound into an organic phase which is finally separated.

59%

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SKU	FT2002-20
Price:	$104.00 (€ 81.85)

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Extract the pure compound with water and ethyl acetate/heptane

Extract the pure compound with water and chloroform