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eChemShop - Chemical Reagents, Natural Compounds, Software, HPLC columns :: dPEG™ Reagents :: New dPEG™ Products :: NHS-dPEG™4-S-S-Biotin

NHS-dPEG™4-S-S-Biotin

Attributes

Reactivity 1 amine
Solubility Methylene chloride or DMAC and DMSO
Molecular Weight 751.94

Special Notes

• Amine reactive biotinylation reagent
• Disulfide cleavable; replaces an inferior sulfo-NHS-S-S-biotin
• Very water soluble, hydrophilic and eliminates non-specific binding
• deal spacer length for binding streptavidin conjugates; chain length from amide to terminal carbonyl is 28.7 Angstroms and 24 atoms
• dPEG™ linker is designed to be the same length as the LC-LC spacer, which is ideal for the streptavidin binding pocket
• Non-antigenic and non-immunogenic spacer arm
• ELIMINATES aggregation and precipitation when labeling antibodies and other biological materials (a severe problem with conventional biotinylation reagents)

Protocols

Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). Use protocol on page 377, except make a 40 mg/ml solution of the NHS-biotin in dry DMF. The use of dry DMF is critical. This can easily be obtained by placing 3 Angstrom or 4 Angstrom molecular sieves into reagent grade DMF, shaking and letting stand at least overnight. If possible handle the NHS-biotin under an inert atmosphere. We find that handling these materials in an Atmosbag, available from Sigma-Aldrich, under argon (preferred) or nitrogen, is the best, most convenient and inexpensive means of handling these moisture sensitive materials. The remaining solid NHS-biotin should be stored under an inert gas (argon preferred) at about 4 Celsius. The product can be pre-dissolved in an organic solvent OR can be directly dissolved in your reaction medium based on its inherent water solubility.

Spacer length (same as LC-LC spacer) is optimal for rapid and tight avidin/streptavidin binding. Hydrophilic spacer arm eliminates non-specific binding that causes aggregation and precipitation problems, which commonly occur when labeling antibodies and other biological materials. Hydrophilic dPEG™ spacer is non-immunogenic. Ultra high purity.

References

Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). See pages 377-380.

59%

Details


SKU	BQ10194-50
Weight	2.00 lbs
Price:	$175.00 (€ 137.72)

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