| CAS #: 1264662-85-2 Amount: 25mg  
Item Name: Biotin-dPEG®3-TFPA Item #: 10308 Mol. Wt.: 663.69; single compound dPEG® Spacer is 15 atoms and 16.9 A Product Features and Benefits: FIRST TRULY NON-SPECIFIC PHOTOREACTIVE biotinylation reagent for non-specific labeling of ANY organic containing compounds, surfaces or particles. It has the perfect spacer arm that contains a hydrophilic, non-immunogenic dPEG® pegylation spacer arm Also, the dPEG® pegylation spacer will reduce or eliminate issue of aggregation and non-specific binding (noise) typical of convention LC spacers…it is PERFECT!! Advantages of tetrafluorophenyl azide as a photoactivating group include the following: Reacts with C-H and N-H bonds non-specifically from the photogenerated singlet nitrene with high efficiencies. Highly efficient incorporation Historical perspective: It has been claimed for many, many years that the commercially available phenyl azides are non-specific. This is INCORRECT!! They are not only very, very inefficient, but are also completely specific for amines. The perfluorophenyl azides have ideal photophysical properties, and have been designed to overcome the deficiencies in these old reagents. References: Excellent references for the tetrafluorophenyl azides include the following: Chemistry and Kinetics of Singlet (Pentafluorophenyl)nitrene, M. S. Platz, et al., J. Amer. Chem. Soc., 11IB4, 5054-5067 (1992). Synthesis and Binding of New Polyfluorinated Aryl Azides to alpha-Chymotrypsin. New Reagents for Photoaffinity Labeling. M. S. Platz, et al., Bioconjugate Chemistry, 4,256-261 (1993). Synthesis of a Tetrafluoro-Substituted Aryl Azide and Its Protio Analogue as Photoaffinity Labeling Reagents for the Estrogen Receptor, J.A. Katzenellenbogen, et al., J. Org. Chem., 56, 3125-3133 (1991). NHS Ester Functionalized Perfluorphenyl Azides as Novel Photoactive Heterobifunctional Cross-linking Reagents. J.F.W. Keana, et al., Bioconjugate Chemistry, 5, 151-157 (1994). |
