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  eChemShop - Chemical Reagents, Natural Compounds, Software, HPLC columns :: Molecular Biology

    Molecular Biology
 

EZ-10 96-Well Spin Column PCR* Products Purification Kit**

For automated purification of PCR products and DNA cleanup of variable enzymatic reactions.

Components BS365, 5 Plates BS366, 10 Plates


Reproducible yields of PCR products from a single sample by using the Kit.

Lane 1: DNA marker. Lanes 2-5: The crude PCR products Lanes 6 to 25 are the purified PCR products. Recovered yields are approximately 80%.
EZ-10 96-Well Plate 5 10
Deep Well Collection Plate 10 20
96 storage plate 5 10
Binding Buffer I 150ml 300ml
Wash Solution 2x60 ml 4x60 ml
Elution Buffer 30 ml 60 ml
Sealing film 20 40
Complete Protocol 1 1
Price 420.00 750.00
Application:

1) Recovery of PCR* products from reaction solution
2) DNA Cleanup from the enzymatic reactions
3) Removal of nucleotides and primers (<40-mer)

Features:

 1) Rapid and Economic.
2) High yields (60-80%). It is suitable to recover 100 bp-40 kb DNA fragments.
3) Efficient removal of contaminants. Purified DNA can be used in any downstream applications such as sequencing, labeling, restriction enzymatic digestions, ligations, hybridization, or transformations.
4) No phenol / chloroform extraction or ethanol precipitation

Quality Control:
All components of the kit are tested in purification of PCR* products from PCR* mixture.
Procedure:

The kit uses a simple bind-wash-elute procedure. PCR* products or enzymatic reactions are mixed with the appropriate binding buffer and pass through to 96 columns of the plate. DNA binds to the column. Impurities such as enzymes, salts, nucleotides and primers ( < 40-mer) are washed away. Pure DNA is eluted in a small volume of the low-salt elution buffer or water, ready for use in any downstream applications.

** This Kit has same the contents as EZ-10 96-well Spin Column DNA Cleanup Kit. So this Kit can also be used for 96-well format DNA cleanup.

* The Polynucleotide Chain Reaction (PCR) is covered by patents owned by Hoffman-La Roche Inc



 

EZ-10 96 Well Spin Column Plate DNA Cleanup Minipreps Kit

For automated purification of PCR products and DNA cleanup of variable enzymatic reactions including cDNA synthesis, ligation, restriction digestions, tailing, PCR, alkaline phosphatase, nick translation, due terminators.

369, 370


Components BS369, 5 Plates BS370, 10 Plates


Reproducible yields of high quality of PCR products from a single sample by using EZ-10 96-well Spin Column Plate DNA Cleanup Kit.
EZ-10 96-Well Plate 5 10
Deep Well Collection Plate 10 20
96 storage plate 5 10
Cleanup Solution 150ml 300ml
Wash SolutionA 2x60 ml 4x60 ml
Elution BufferB 30 ml 60 ml
Sealing film 20 40
Complete Protocol 1 1
Price 420.00 750.00
Application:

1) Recovery of PCR* products from reaction solution
2) DNA Cleanup from the enzymatic reactions
3) Removal of nucleotides and primers (<40-mer)

Features:

 1) Rapid and Economic.
2) High yields (60-80%). It is suitable to recover 100 bp-40 kb DNA fragments.
3) Efficient removal of contaminants. Purified DNA can be used in any downstream applications such as sequencing, labeling, restriction enzymatic digestions, ligations, hybridization, or transformations.
4) No phenol / chloroform extraction or ethanol precipitation

Quality Control:
All components of the kit are tested in purification of PCR* products from PCR* mixture.
Procedure:

The kit uses a simple bind-wash-elute procedure. PCR* products or enzymatic reactions are mixed with the appropriate binding buffer and pass through to 96 columns of the plate. DNA binds to the silica-based membrane. Impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA is eluted in a small volume of the low-salt elution buffer or water, ready for use in any downstream applications.

The Polynucleotide Chain Reaction (PCR) is covered by patents owned by Hoffman-La Roche Inc


 

 
  		 
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