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eChemShop - Chemical Reagents, Natural Compounds, Software, HPLC columns :: Rapid Stain CBB Kit
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Rapid Stain CBB Kit |
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| Staining
| Rapid Stain CBB Kit (Coomassie R-250) |
| Quick: |
staining and destaining are completed within 30 minutes |
| Simple: |
just mix the kit components with purified water |
| Highly sensitive: |
detect as little as 20 ng protein |
| Application |
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Conditions
| Gel : |
12.5% Polyacrylamide Gel (Laemli) |
| Buffer solution : |
Tris/Glycine buffer solution |
| Electrophoresis : |
30 mA constant current, 50min |
Sample
| Human Serum |
| Lane1 : marker |
Lane 7 : 250 ng |
| Lane2 : 5,000 ng |
Lane 8 : 100 ng |
| Lane3 : 2,500 ng |
Lane 9 : 70 ng |
| Lane4 : 1,000 ng |
Lane10 : 40 ng |
| Lane5 : 750 ng |
Lane11 : 20 ng |
| Lane6 : 500 ng |
Lane12 : marker |
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| Composition |
A solution : contains acetic acid
B solution : contains ethanol and Coomassie Brilliant Blue R-250
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