COSMOSIL HILIC*NEW*
COSMOSIL HILIC is a new column for hydrophilic interaction chromatography with Triazole
bonded silica packing material. The hydrophilic interaction chromatography is a variation of
normal phase chromatography where a polar stationary phase is used with a mobile phase which
contains a high concentration of organic solvent and a low concentration of aqueous eluent.
The main retention mechanism is the partitioning of the polar analytes between the polar stationary
and the non-polar mobile phase. As it is also called "aqueous normal phase", the elution order is
similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity.
Without using ion-pair reagent COSMOSIL HILIC retains highly polar analytes that would not be
retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with
the positively charged stationary phase, thus acidic compound is strongly retained.
Material Characteristics:
| COSMOSIL HILIC |
| Silica gel |
high purity porous spherical silica gel |
| Average particle size |
5µm |
| Average pore size |
approx. 120 nm |
| Specific surface area |
approx. 300m˛/g |
| Stationary phase |
Triazole |
| Main interaction |
Hydrophilic interaction |
Comparison with C18 column :
COSMOSIL HILIC can separate Glycine and Glycylglycine without ion-pair reagent. Although
C18 column can separate them with ion-pair reagents, there are some disadvantages such as
column equilibration, preparation of mobile phase and column deterioration.
Different interactions :
The retention Mechanism of COSMOSIL HILIC is the combination of hydrophilic interaction
and anion exchange, and the retention can be controlled by changing the mobile phase.
Application
Combination with C18 columns
| Selection of mobile phase |
| Following are the recommended mobile phases for different samples. |
| Acetonitrile / Water = 90 / 10 |
| Acetonitrile / 10mmol/l CH3COONH4 = 90 / 10 |
| Acetonitrile / 10mmol/l CH3COONH4 = 70 / 30 |
| Acetonitrile / 10mmol/l CH3COONH4 = 50 / 50 |
(not eluted)  Acetonitrile / 10mmol/l Phosphatase buffer (pH7.0) = 50 / 50 |
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