| CAS #: 35013-72-0 Amount: 100mg  
Item Name: NHS-biotin Item #: 10205 Mol. Wt.: 341.38; single compound Product Features and Benefits: Amine reactive label Quanta’s exclusive synthetic process produces the highest quality commercial material Efficient label for certain peptides and nucleic acids Critical and versatile building block for Permeates cell membranes Protocol: Hermanson, pg. 512, except make a 40 mg/ml solution of the NHS-biotin in dry DMF. The use of dry DMF is critical. This can easily be obtained by placing 3 Angstrom or 4 Angstrom molecular sieves into reagent grade DMF, shaking and letting stand at least overnight. If possible handle the NHS-biotin under an inert atmosphere. We find that handling these materials in an Atmosbag, available from Sigma-Aldrich, under argon (preferred) or nitrogen, is the best, most convenient and inexpensive means of handling these moisture sensitive materials. The remaining solid NHS-biotin should be stored under an inert gas (argon preferred) at about 4°C. General Biotin/Biotinylation References: Wilchek and Bayer: “Avidin-Biotin Technology”, Methods in Enzymology, Vol. 184, M. Wilchek and E. A. Bayer, eds., 1990, Academic Press, NY. Hermanson: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). . Note: This reference is an extremely practical and informative volume from an experienced practitioner. |
