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10856 SPDP-dPEG 16-acid

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CAS: 1334177-97-7

Item Name: SPDP-dPEG 16-acid

Item #: 10856

Mol. Wt.: 990.22;

single compound dPEG Spacer is 59 atoms and 63.7 A approx.

10856 SPDP-dPEG 16-acid

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$275.00

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CAS: 1334177-97-7
Item Name: SPDP-dPEG 16-acid
Item #: 10856
Mol. Wt.: 990.22;
single compound dPEG Spacer is 59 atoms and 63.7 A approx.
Product Features and Benefits: Unique dPEG - containing HETEROBIFUNCTIONAL pegylation crosslinker reagent with amine and sulfhydryl/thiol reactivity. The carboxylic acid needs to be activated in situ with reagents like EDC/NHS and DCC/HOBt, NHS or PFP. Cleavable SPDP pegylation reagents produce a disulfide bond with thiols, a bond which can later be cleaved with a variety of reducing agents, like DTT or TCEP. Measure initial dPEG SPDP incorporation and then follow the conjugation at 343 nm Replace the hydrophobic LC-SPDP with this SPDP pegylation reagent with vastly superior properties and performance of the dPEG . Spacer will reduce or eliminate problems with aggregation and immunogenicity typically significant, but ignored issues with conventional heterobifunctionals Diagnostic applications: Expect dramatic increase in S/N ratios in analytical applicationsdecreased noisereduces non-specific binding/aggregation Applications: The general application of this class of heterobifunctional pegylation crosslinkers is the controlled and selective conjugation of an amine- containing target (reacts with the NHS ester), then subsequently with a sulfhydryl containing complementary target molecule to form another disulfide. Many biological molecules contain the amine function AND the complementary compounds, like peptides, oligos or other biologicals can be terminated with thiols or have thiols designed into them, and vise versa. The contrast with the maleimide containing crosslinkers is that the SPDP derivatives form a disulfide, stable under non-reducing conditions or of other thiols, but can be cleaved with a reducing agent, or exchanged with another thiol. Also, having the SPDP presents the potential of using the pyridine-2-thione, released in the reaction with another thiol, to measure the level of the dPEG SPDP incorporation in the labeling step or to monitor the subsequent reaction with another thiol by measuring its absorption at 343 nm. The dPEG SPDP pegylation reagents offer tremendous flexibility in the range of sizes of the discrete pegylation spacers, all of which give the incorporation of the very hydrophilic/water soluble and non-immunogenic dPEG pegylation spacer. All the positives of the conventional reagents is retained, but with the addition of the perfect properties and variable options in the dPEG containing SPDP pegylation reagents.
References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0)., See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Gregs extensive index on pg. 1192-1193 for references to a number and range of applications and their respective protocols.
Protocol: Some representative examples of the range of possible dPEG SPDP applications from Bioconjugate Chemistry. M. Zhao, et al., Bioconjugate Chemistry, 13, 840-844 (2002) D.H.Na, et al., Bioconjugate Chemistry, 10, 306-310 (1999) A. S. Kamruzzahan, et al., Bioconjugate Chemistry, 17, 1473 (2006) A. K. Patri, et al., Bioconjugate Chemistry, 15, 1174-1181 (2004) H. J. Gruber, et al., Bioconjugate Chemistry, 11, 161-166 (2000) N. J. Davimandan, et al., Bioconjugate Chemistry, 17, 1376-1384 (2006) M. Heggli, et al., Bioconjugate Chemistry, 14, 967-973 (2003) S. B. Rajur et al., Bioconjugate Chemistry, 8, 935-940 (1997) All of these applications, and any 158 using dPEG based heterobifunctional pegylation reagents will benefit from having the dPEG spacer, for all the reasons listed in
Product Features and Benefits.