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10064 MAL-dPEG 6-NHS ester

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CAS: 1137109-21-7

Item Name: MAL-dPEG 6-NHS ester

Item #: 10064

Mol. Wt.: 601.60;

single compound dPEG Spacer is 28 atoms and 31.7 A

10064 MAL-dPEG 6-NHS ester

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$215.00

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CAS: 1137109-21-7
Item Name: MAL-dPEG 6-NHS ester
Item #: 10064
Mol. Wt.: 601.60;
single compound dPEG Spacer is 28 atoms and 31.7 A
Product Features and Benefits: Unique dPEG -containing HETEROBIFUNCTIONAL pegylation crosslinker reagent with amine and sulfhydryl/thiol reactivity Activate the carboxyl in situ with EDC/NHS or other activation chemistry of the carboxylic acid Replacement for MBS or sulfo-smcc in standard conjugations with far superior performance Pegylation spacer will reduce or eliminate problems with aggregation and immunogenicity typical, though under appreciate, issues with conventional heterobifunctionals See dramatic increase in your S/N ratiosdecreased noisereduces non-specific binding interactions Product is also designed also to be used to activate carrier proteins in place of SMCC, sulfo-SMCC or MBS for conjugating hapten, e.g., peptide, to a carrier, e.g., KLH, BSA, OVA, etc., in order to reduce or eliminate the antigenicity and specificity issues with conventional activating crosslinkers, which are reflected in the properties of the resulting antibodies Much higher functional loadings can be achieved due to the increased water solubility imparted by the peglation reagent to the carrier hapen conjugate, with decreased immunogenicity towards crosslinker and carrier Applications: The general application of this class of heterobifunctional pegylation crosslinkers is the controlled and selective conjugation of an amine- containing target (reacts with the activated acid), then subsequently with a sulfhydryl containing complementary target molecule (reacts with the maleimide). Many biological molecules contain the amine function AND the complementary compounds, like peptides, oligos or other biologicals can be terminated with thiols or have thiols designed and engineered into them. Specific examples of the use of the MAL-dPEG x-NHS ester heterobifunctionals include Hapten-Carrier conjugation, Enzyme-antibody conjugation and protein-Avidin/Streptavidin conjugation. The chief advantage of the dPEG -containing pegylation heterobifunctional crosslinkers over the conventional ones like SMCC/sulfo-SMCC, MBS, EMCS, etc., is the incorporation of the very hydrophilic/water soluble and non-immunogenic pegylation spacer. The extreme hydrophobicity of conventional reagents has made them a challenge to apply, and non-optimal solutions, with no design options. The thread of the conventional linkers has been to shorter spacers to get around some issues of antigenicity and poor water solubility, but most conjugation applications are screaming for longer spacers, our dPEG series of pegylation reagents provides the ideal NEW options in heterobifunctional crosslinking
Protocol: a) Hapten-Carrier conjugation, pp. 788; b) Liposome conjugation, 871, and antibody liposome conjugation, pg. 883. All of these applications, and any 158 using dPEG based heterobifunctional pegylation reagents will benefit from having the dPEG spacer, for most or all of the reasons listed in
Product Features and Benefits. For the present dPEG products it is probably still best to use an organic solvent like DMAC, DMF or DMSO to ensure complete solubilization and homogeneity of the crosslinker in the crosslinking buffer system. If water or buffer is preferred, use the standard precautions related to the stabilities of both NHS ester and maleimide, by maintaining a pH between 7.2 and 7.5.
References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0)., See pp. 276-335 for general description and use of heterobifunctional crosslinkers, as well as his specific discussion with protocols of our MAL-dPEG x-NHS esters on pp. 718-722.