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Staining

 

 

  • Conditions

     

    Gel : ReadyGel 4-20%
    Buffer solution : Tris/Glycine/SDS buffer solution (400ml)
    Electrophoresis : 80 mA (constant voltage)
    Staining : 1 hr.

     

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    CBB Stain One (Ready To Use) (Coomassie G-250)
    Ready to use : one solution in one bottle
    Fast and convenient : use only water for destaining
    Safe : no hazardous methanol or acetic acid
    Highly sensitive : detects proteins at nanogram level
    Application

     

  • Sample
    Human Serum
    Lane1 : E coli proteins (SDS, with 2ME buffer extraction) 5 µl
    Lane2 : E coli proteins (SDS, with 2ME buffer extraction) (10 time dilution) 5 µl
    Lane3: protein markers (1.7 µg/µl) 2 µl
    Lane4: protein markers (550 ng/µl) 2 µl
    Lane5: protein markers (170 ng/µl) 2 µl
    Lane6: protein markers (55 ng/µl) 2 µl