m-dPEG®x-amine

Amount: 100mg

Item Name: m-dPEG®4-amine

Item #: 10175

Mol. Wt.: 207.27;

single compound dPEG® Spacer is 14 atoms and 15.5 A

Product Features and Benefits: Carbonyl/carboxyl reactive dPEG®; this unique pegylation reagent reacts with acids, active esters and aldehydes. Each is a single compound; made from 99.5% pure tetraethylene glycol In modification reactions: Pegylation arm imparts significant and surprising water solubility, and the modifier itself is non-immunogenic and non-toxic, so can decrease these properties in the modified application For surface applications: m-dPEG® amine pegylation reagent will react on surfaces to significantly reduce or eliminate non-specific interactions and create a monolayer surface structure. Can be used in conjunction with the amino dPEG™ acids or amino dPEG® esters to protect the surface as well as to impart functionality back to a carboxylated surface.

Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications. General guidelines for use: In all cases care should be taken in handling the materials, they are amines. All are very hygroscopic and will react with the atmosphere over time, so should be handled accordingly. For weighing purposes, dPEG®x, x < 12 can be handled as liquids, x > 12 are nice solids when kept cool or cold. X = 12 can be easily melted and treated as a liquid. Again, these are amines and should be treated accordingly. Organic media reactions: The amines are soluble in all organic solvents of moderate to higher polarity. We prefer methylene chloride or DMAC. The amines are easily reacted with acids activated in situ or with active esters, like HOBt (and derivatives), PFP or NHS, using a tertiary amine to help drive the reaction to completion. Aqueous media: You can activate your acid with EDC and sulfo-NHS, then react with the m-dPEG® amine pegylation reagent. This is best done in a non-amine containing buffer, e.g., in MES buffer at pH 5-6, then raise the pH to between 7.2 to 7.5 with phosphate buffer just before reacting with the amine or after the amine has been added. See Greg’s chapter 3, pp. 215-233 for more details and sample protocols.

Amount: 100mg

Item Name: m-dPEG®12-amine

Item #: 10288

Mol. Wt.: 559.69;

single compound dPEG® Spacer is 38 atoms and 43.9 A

Product Features and Benefits: Carbonyl/carboxyl reactive dPEG®; this unique pegylation reagent reacts with acids, active esters and aldehydes. Each is a single compound; made from 99.5% pure tetraethylene glycol In modification reactions: Pegylation arm imparts significant and surprising water solubility, and the modifier itself is non-immunogenic and non-toxic, so can decrease these properties in the modified application For surface applications: m-dPEG® amine pegylation reagent will react on surfaces to significantly reduce or eliminate non-specific interactions and create a monolayer surface structure. Can be used in conjunction with the amino dPEG® acids or amino dPEG® esters to protect the surface as well as to impart functionality back to a carboxylated surface.

Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications. General guidelines for use: In all cases care should be taken in handling the materials, they are amines. All are very hygroscopic and will react with the atmosphere over time, so should be handled accordingly. For weighing purposes, dPEG®x, x < 12 can be handled as liquids, x > 12 are nice solids when kept cool or cold. X = 12 can be easily melted and treated as a liquid. Again, these are amines and should be treated accordingly. Organic media reactions: The amines are soluble in all organic solvents of moderate to higher polarity. We prefer methylene chloride or DMAC. The amines are easily reacted with acids activated in situ or with active esters, like HOBt (and derivatives), PFP or NHS, using a tertiary amine to help drive the reaction to completion. Aqueous media: You can activate your acid with EDC and sulfo-NHS, then react with the m-dPEG® amine pegylation reagent. This is best done in a non-amine containing buffer, e.g., in MES buffer at pH 5-6, then raise the pH to between 7.2 to 7.5 with phosphate buffer just before reacting with the amine or after the amine has been added. See Greg’s chapter 3, pp. 215-233 for more details and sample protocols.

Amount: 100mg

Item Name: m-dPEG®24-amine

Item #: 10318

Mol. Wt.: 1088.32;

single compound dPEG® Spacer is 74 atoms and 86.1 A

Product Features and Benefits: Carbonyl/carboxyl reactive dPEG®; this unique pegylation reagent reacts with acids, active esters and aldehydes. Each is a single compound; made from 99.5% pure tetraethylene glycol In modification reactions: Pegylation arm imparts significant and surprising water solubility, and the modifier itself is non-immunogenic and non-toxic, so can decrease these properties in the modified application For surface applications: m-dPEG® amine pegylation reagent will react on surfaces to significantly reduce or eliminate non-specific interactions and create a monolayer surface structure. Can be used in conjunction with the amino dPEG® acids or amino dPEG® esters to protect the surface as well as to impart functionality back to a carboxylated surface.

Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications. General guidelines for use: In all cases care should be taken in handling the materials, they are amines. All are very hygroscopic and will react with the atmosphere over time, so should be handled accordingly. For weighing purposes, dPEG®x, x < 12 can be handled as liquids, x > 12 are nice solids when kept cool or cold. X = 12 can be easily melted and treated as a liquid. Again, these are amines and should be treated accordingly. Organic media reactions: The amines are soluble in all organic solvents of moderate to higher polarity. We prefer methylene chloride or DMAC. The amines are easily reacted with acids activated in situ or with active esters, like HOBt (and derivatives), PFP or NHS, using a tertiary amine to help drive the reaction to completion. Aqueous media: You can activate your acid with EDC and sulfo-NHS, then react with the m-dPEG® amine pegylation reagent. This is best done in a non-amine containing buffer, e.g., in MES buffer at pH 5-6, then raise the pH to between 7.2 to 7.5 with phosphate buffer just before reacting with the amine or after the amine has been added. See Greg’s chapter 3, pp. 215-233 for more details and sample protocols.

Amount: 100mg

Item Name: m-dPEG®48-amine

Item #: 10918

Mol. Wt.: 2310.73;

single compound dPEG® Spacer is 146 atoms and 174.0 A

Product Features and Benefits: Carbonyl/carboxyl reactive dPEG®; this unique pegylation reagent reacts with acids, active esters and aldehydes. Each is a single compound; made from 99.5% pure tetraethylene glycol In modification reactions: Pegylation arm imparts significant and surprising water solubility, and the modifier itself is non-immunogenic and non-toxic, so can decrease these properties in the modified application For surface applications: m-dPEG® amine pegylation reagent will react on surfaces to significantly reduce or eliminate non-specific interactions and create a monolayer surface structure. Can be used in conjunction with the amino dPEG™ acids or amino dPEG® esters to protect the surface as well as to impart functionality back to a carboxylated surface.

Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications. General guidelines for use: In all cases care should be taken in handling the materials, they are amines. All are very hygroscopic and will react with the atmosphere over time, so should be handled accordingly. For weighing purposes, dPEG®x, x < 12 can be handled as liquids, x > 12 are nice solids when kept cool or cold. X = 12 can be easily melted and treated as a liquid. Again, these are amines and should be treated accordingly. Organic media reactions: The amines are soluble in all organic solvents of moderate to higher polarity. We prefer methylene chloride or DMAC. The amines are easily reacted with acids activated in situ or with active esters, like HOBt (and derivatives), PFP or NHS, using a tertiary amine to help drive the reaction to completion. Aqueous media: You can activate your acid with EDC and sulfo-NHS, then react with the m-dPEG® amine pegylation reagent. This is best done in a non-amine containing buffer, e.g., in MES buffer at pH 5-6, then raise the pH to between 7.2 to 7.5 with phosphate buffer just before reacting with the amine or after the amine has been added. See Greg’s chapter 3, pp. 215-233 for more details and sample protocols.