10219 Biotin-dPEG®4-hydrazide

Amount: 50mg

Item Name: Biotin-dPEG®4-hydrazide

Item #: 10219

Mol. Wt.: 505.63;

single compound dPEG® Spacer is 18 atoms and 20.6 A

Product Features and Benefits: Carbonyl reactive biotinylation reagent with a dPEG®4 pegylation spacer Reacts with aldehydes and ketones to give stable hydrazones in a single step. Also reacts with activated carboxylic acids Physical properties are similar to those of the NHS-dPEG®4-biotin, helping to prevent or eliminate aggregation when labeling large biological, and products shows less non-specific binding then conventionally biotinylated compounds 20.6 Angstrom pegylation spacer arm (length from amide carbonyl to formed hydrazone N) Solubility in water: >50% w/v ! Protocols: Ref.: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). See pp. 733-736 for a general discussion of the chemistry and a general protocol for labeling of a glycoprotein is given on pg. 736.