| CAS #: 1334172-62-1 Amount: 100mg  
Item Name: NHS-dPEG®4-Lys-(dPEG®4-biotin)2 Item #: 10360 Mol. Wt.: 1437.72; single compound dPEG® Spacers are 40 and 35 atoms and 43.4 and 41.7 A, resp. Product Features and Benefits: Amine reactive bis biotinylation pegylation reagent with a dPEG® spacer arm with potential to polymize avidin or streptavidin at the label site. Very water soluble, hydrophilic and eliminates non-specific binding. Ideal spacer length for binding streptavidin conjugates total dPEG® length short enough to bend just one streptaviden subunit. Non-antigenic and non-immunogenic spacer arm Also ELIMINATES aggregation and precipitation when labeling antibodies and other biological materials (a severe problem with conventional biotinylation reagents) Protocol: See Hermanson (reference), pg. 728 for the NHS-dPEG®4 biotin. While this pegylation reagent can be used directly in water, this should, as the protocol calls, be dissolved in an organic solvent for optimal and easiest application Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications. | 
