| CAS #: 1334172-61-0 Amount: 100mg  
Item Name: NHS-dPEG®4-Biotinidase resistant biotin Item #: 10202 Mol. Wt.: 673.78; single compound dPEG® Spacer is 19 atoms and 21.5 A Product Features and Benefits: Amine reactive biotinylation pegylation reagent with a dPEG®4 spacer…perfect length for most applications. BIOTINIDASE RESISTANT…vital especially for in vivo work or clinical analysis. Very water soluble, hydrophilic and eliminates non-specific binding. This reagent gives the spacer length with the pegylation arm for optimal biotin binding with streptavidin conjugates; chain length from amide to terminal carbonyl is 19.2 Angstroms. Designed to be the same length as the LC-LC spacer, which is ideal for the streptavidin binding pocket. Non-antigenic, non-immunogenic and water soluble pegylation spacer arm ELIMINATES non-specific binding issues, things like aggregation and precipitation when labeling antibodies and other biological materials (a severe problem with conventional biotinylation reagents). EXPECT GREAT, even dramatic S/N increases! Protocol: The pegylation reagent can be pre-dissolved in an organic solvent OR can be directly dissolved in pure water, but must be used immediately. The reaction is best run between pH 7.2 and 8 using a non-amine buffer, e.g., PBS pH 7.2. Typical incubation times will be about 2 hours on ice and 30 minutes at RT. For large molecules the unreacted biotinylation reagent can be removed with gel filtration or dialysis. References: D. Scott Wilbur, et al., “Biotin Reagents for Antibody Pretargeting. 7. Investigation of Chemically Inert Biotinidase Blocking Functionalities for Synthetic Utility,” Bioconjugate Chemistry, 17(6), 1514-1522 (2006), and references therein. Plus Scott’s earlier papers in this area: a) Bioconjugate Chemistry, 12, 616-623 (2001) and b) Bioconjugate Chemistry, 8, 572-584 (1997). |
