dPEG®x-biotin acid

Amount: 100mg

Item Name: dPEG®4-biotin acid

Item #: 10199

Mol. Wt.: 491.60;

single compound dPEG® Spacer is 16 atoms and 19.2 A

Product Features and Benefits: Imparted physical properties are the biotin same as the dPEG®4 NHS esters (10200, 10198); the labeled compound becomes very hydrophilic because of the dPEG® pegylation spacer, making the biotin very available for streptavidin binding in the capture/binding step. The 10199 pegylation spacer arethe same length as 10200 and 10198, resp., and its length is optimal for rapid and tight avidin/SA binding properties. Very water soluble, hydrophilic and eliminates non-specific binding. 10198 has a LONGER 47.6 Angstrom spacer: the longer pegylation arm give ready accessibility to the biotin by the streptavidin/avidin conjugate binding; also where the amine of the biotinylation site may be buried, the longer arm is ideal for giving accessibility to the binding conjugates. Non-antigenic and non-immunogenic spacer arm The dPEG® pegylation spacer and its amazing properties ELIMINATES aggregation and precipitation when labeling antibodies and other biological materials, and will also significantly increase S/N in analytical applications as well. Protocol for in situ activation to the NHS ester: Use a 10-20% molar excess of EDC and NHS in dry methylene chloride (dried over 3A molecular sieves). Add a methylene chloride solution of the acid to the dry reagents under dry conditions. Stir for several hours or overnight, then evaporate the solvent and use. Can also treat reaction mixture with a small amount of silica gel to adsorb the excess EDC and the urea by-product, filter, then evaporate the solvent and use.

Note of caution: The NHS should be added with the EDC to prevent formation of the anhydride. Also, can be used for peptide labeling using a variety of activating “concoctions,” which can also be used for direct coupling to other amines.

Protocol: See Hermanson pg. 728 for a typical protocol for a antibody or protein biotinylation using an in situ activated form of 10197. Generally customers use this when they have their favorite way of activating the acid, as opposed to using our dPEG®12 pegylation spacer containing biotin reagent. The reagent can be pre-dissolved in an organic solvent OR can be directly dissolved in your reaction medium based on its inherent water solubility. Also, see above in situ activation procedure.

Amount: 100mg

Item Name: dPEG®48-biotin acid

Item #: 10776

Mol. Wt.: 2500.99;

single compound dPEG® Spacer is 157 atoms and 187.8 A

Product Features and Benefits: Imparted physical properties are the biotin same as the dPEG®4 NHS esters (10200, 10198); the labeled compound becomes very hydrophilic because of the dPEG® pegylation spacer, making the biotin very available for streptavidin binding in the capture/binding step. The 10199 pegylation spacer arethe same length as 10200 and 10198, resp., and its length is optimal for rapid and tight avidin/SA binding properties. Very water soluble, hydrophilic and eliminates non-specific binding. 10198 has a LONGER 47.6 Angstrom spacer: the longer pegylation arm give ready accessibility to the biotin by the streptavidin/avidin conjugate binding; also where the amine of the biotinylation site may be buried, the longer arm is ideal for giving accessibility to the binding conjugates. Non-antigenic and non-immunogenic spacer arm The dPEG® pegylation spacer and its amazing properties ELIMINATES aggregation and precipitation when labeling antibodies and other biological materials, and will also significantly increase S/N in analytical applications as well. Protocol for in situ activation to the NHS ester: Use a 10-20% molar excess of EDC and NHS in dry methylene chloride (dried over 3A molecular sieves). Add a methylene chloride solution of the acid to the dry reagents under dry conditions. Stir for several hours or overnight, then evaporate the solvent and use. Can also treat reaction mixture with a small amount of silica gel to adsorb the excess EDC and the urea by-product, filter, then evaporate the solvent and use.

Note of caution: The NHS should be added with the EDC to prevent formation of the anhydride. Also, can be used for peptide labeling using a variety of activating “concoctions,” which can also be used for direct coupling to other amines.

Protocol: See Hermanson pg. 728 for a typical protocol for a antibody or protein biotinylation using an in situ activated form of 10197. Generally customers use this when they have their favorite way of activating the acid, as opposed to using our dPEG®12 pegylation spacer containing biotin reagent. The reagent can be pre-dissolved in an organic solvent OR can be directly dissolved in your reaction medium based on its inherent water solubility. Also, see above in situ activation procedure.