m-dPEG®x-alcohol

Amount: 1000mg

Item Name: m-dPEG®4-alcohol

Item #: 10348

Mol. Wt.: 208.25;

single compound dPEG® Spacer is 14 atoms and 46.3 A

Product Features and Benefits: Potential applications for these dPEG® pegylation reagents include; i) surface modification reagent; ii) solubilizing agent; or iii) blocking/capping reagent. Will reduce or eliminate non-specific interactions, aggregation, reduces immunogenicity, increases water solubility, among other great advantages. The hydroxyl can be converted to a wide variety of functionality which we may not currently offer in our catalog or on our website!

Protocol: The following is a sample protocol for pre- or in situ activation of the free alcohol. To a 10% w/v solution* of the m-dPEG® alcohol in dry methylene chloride is added 1.0 eq. of DSC as the solid. To this solution is added drop wise a methylene chloride solution of triethylamine** (1.0 eq., purified and dry). The solution is stirred at room temperature for 8 hours (overnight is convenient too). The reaction can be conviently monitored by TLC (Methenal ; theyl acetate; acetic acid; NHS ester quences, both develop very strongly to iodine vapor.) The methylene chloride is removed under reduced pressure along with any residual triethylamine. The resulting activated ester can be used as is. (Note: To get total conversion, one could use excesses of the DSC and TEA. However, residual DSC can potentially activate other functionality in subsequent chemistry and further purification would be required.) *Solvents that can be used can include: Methylene chloride (our preferred), DMF, DMAC, acetonitrile, THF **Bases: Others like DMAP (4-dimethylaminopyridine) and pyridine can also be used. Also, the alcohol OH can easily be converted to other convenient leaving groups such as the tosylate or mesylate using the corresponding sulfonyl chloride, triethylamine in methylene chloride. These leaving groups can then be displaced with a nucleophile of choice, such as amine, S- (thiolate) and O- (alkoxide), among others.

Amount: 100mg

Item Name: m-dPEG®15-alcohol

Item #: 10894

Mol. Wt.: 692.83;

single compound dPEG® Spacer is 46 atoms and 54.7 A

Product Features and Benefits: Potential applications for these dPEG® pegylation reagents include; i) surface modification reagent; ii) solubilizing agent; or iii) blocking/capping reagent. Will reduce or eliminate non-specific interactions, aggregation, reduces immunogenicity, increases water solubility, among other great advantages. The hydroxyl can be converted to a wide variety of functionality which we may not currently offer in our catalog or on our website!

Protocol: The following is a sample protocol for pre- or in situ activation of the free alcohol. To a 10% w/v solution* of the m-dPEG® alcohol in dry methylene chloride is added 1.0 eq. of DSC as the solid. To this solution is added drop wise a methylene chloride solution of triethylamine** (1.0 eq., purified and dry). The solution is stirred at room temperature for 8 hours (overnight is convenient too). The reaction can be conviently monitored by TLC (Methenal ; theyl acetate; acetic acid; NHS ester quences, both develop very strongly to iodine vapor.) The methylene chloride is removed under reduced pressure along with any residual triethylamine. The resulting activated ester can be used as is. (Note: To get total conversion, one could use excesses of the DSC and TEA. However, residual DSC can potentially activate other functionality in subsequent chemistry and further purification would be required.) *Solvents that can be used can include: Methylene chloride (our preferred), DMF, DMAC, acetonitrile, THF **Bases: Others like DMAP (4-dimethylaminopyridine) and pyridine can also be used. Also, the alcohol OH can easily be converted to other convenient leaving groups such as the tosylate or mesylate using the corresponding sulfonyl chloride, triethylamine in methylene chloride. These leaving groups can then be displaced with a nucleophile of choice, such as amine, S- (thiolate) and O- (alkoxide), among others.

Amount: 100mg

Item Name: m-dPEG®23-alcohol

Item #: 10942

Mol. Wt.: 1045.25;

single compound dPEG® Spacer is 70 atoms and 83.1 A

Product Features and Benefits: Potential applications for these dPEG® pegylation reagents include; i) surface modification reagent; ii) solubilizing agent; or iii) blocking/capping reagent. Will reduce or eliminate non-specific interactions, aggregation, reduces immunogenicity, increases water solubility, among other great advantages. The hydroxyl can be converted to a wide variety of functionality which we may not currently offer in our catalog or on our website!

Protocol: The following is a sample protocol for pre- or in situ activation of the free alcohol. To a 10% w/v solution* of the m-dPEG® alcohol in dry methylene chloride is added 1.0 eq. of DSC as the solid. To this solution is added drop wise a methylene chloride solution of triethylamine** (1.0 eq., purified and dry). The solution is stirred at room temperature for 8 hours (overnight is convenient too). The reaction can be conviently monitored by TLC (Methenal ; theyl acetate; acetic acid; NHS ester quences, both develop very strongly to iodine vapor.) The methylene chloride is removed under reduced pressure along with any residual triethylamine. The resulting activated ester can be used as is. (Note: To get total conversion, one could use excesses of the DSC and TEA. However, residual DSC can potentially activate other functionality in subsequent chemistry and further purification would be required.) *Solvents that can be used can include: Methylene chloride (our preferred), DMF, DMAC, acetonitrile, THF **Bases: Others like DMAP (4-dimethylaminopyridine) and pyridine can also be used. Also, the alcohol OH can easily be converted to other convenient leaving groups such as the tosylate or mesylate using the corresponding sulfonyl chloride, triethylamine in methylene chloride. These leaving groups can then be displaced with a nucleophile of choice, such as amine, S- (thiolate) and O- (alkoxide), among others.