| CAS #: 1260247-51-5 Amount: 50mg  
Item Name: NHS-S-S-dPEG®4-biotin Item #: 10194 Mol. Wt.: 751.94; single compound dPEG® Spacer is 24 atoms and 28.7 A Product Features and Benefits: Amine reactive CLEAVABLE biotinylation reagent with pegylation spacer for more effective binding, availability and solubility Disulfide is cleavable; replaces an inferior sulfo-NHS-S-S-biotin without the pegylation arm. Very water soluble, hydrophilic and eliminates non-specific binding. Ideal dPEG® pegylation spacer length for binding streptavidin conjugates; chain length from amide to terminal carbonyl is 28.7 Angstroms and 24 atoms. dPEG® linker is designed to be the same length as the LC-LC spacer, which is ideal for the streptavidin binding pocket. Non-antigenic and non-immunogenic pegylation spacer arm ELIMINATES aggregation and precipitation when labeling antibodies and other biological materials (a severe problem with conventional biotinylation reagents) Protocol: See Hermanson (reference), pg. 728 for the NHS-dPEG®4 biotin. While this pegylation reagent can be used directly in water, this should, as the protocol calls, be dissolved in an organic solvent for optimal and easiest application Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications. | 
