NHS-dPEG®x-biotin

Amount: 50mg

Item Name: NHS-dPEG®12-biotin

Item #: 10198

Mol. Wt.: 941.09;

single compound dPEG® Spacer is 40 atoms and 47.6 A

Product Features and Benefits: Amine reactive biotinylation reagent with a dPEG® pegylation spacer arm; activate in situ Very water soluble, hydrophilic and eliminates non-specific binding. Non-antigenic and non-immunogenic spacer arm The dPEG® pegylation spacer and its amazing properties ELIMINATES aggregation and precipitation when labeling antibodies and other biological materials, and will also significantly increase S/N in analytical applications as well. EXPECT GREAT, even dramatic S/N increases! Protocol for in situ activation to the NHS ester: Use a 10-20% molar excess of EDC and NHS in dry methylene chloride (dried over 3A molecular sieves). Add a methylene chloride solution of the acid to the dry reagents under dry conditions. Stir for several hours or overnight, then evaporate the solvent and use. Can also treat reaction mixture with a small amount of silica gel to adsorb the excess EDC and the urea by-product, filter, then evaporate the solvent and use.

Note of caution: The NHS should be added with the EDC to prevent formation of the anhydride. Also, can be used for peptide labeling using a variety of activating “concoctions,” which can also be used for direct coupling to other amines.

Protocol: See Hermanson pg. 728 for a typical protocol for a antibody or protein biotinylation using an in situ activated form of 10197. Generally customers use this when they have their favorite way of activating the acid, as opposed to using our dPEG®12 pegylation spacer containing biotin reagent. The reagent can be pre-dissolved in an organic solvent OR can be directly dissolved in your reaction medium based on its inherent water solubility. Also, see above in situ activation procedure.

Reference: Greg T. Hermanson, Bioconjugate Techniques, 2nd Ed, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-730 in his Chapter 18 on discrete PEG compounds for pegylation applications.